Archivos suplementarios
Palabras clave
Multilocus genotypes
SSR
Genetic diversity
Solanaceae Oomicetos
Genotipos multilocus
SSR
Diversidad genética
Solanáceas
Cómo citar
Resumen
Introducción. Phytophthora infestans sensu lato es el fitopatógeno causante del tizón tardío en solanáceas, con impactos ambientales, económicos y de seguridad alimentaria. Presenta alta incidencia de la enfermedad y cambios genéticos acelerados. Objetivo. Evaluar la variabilidad genética de las poblaciones de P. infestans sensu lato aisladas de solanáceas en municipios del sur de Colombia. Materiales y métodos. Se obtuvieron 40 aislamientos de P. infestans sensu lato de cuatro hospederos de solanáceas provenientes de municipios de Nariño y Putumayo. Se extrajo el ADN y se amplificó con cinco marcadores microsatélites. Las bandas se visualizaron en geles de agarosa al 3% y se midieron con ImageJ. El análisis de datos se realizó con el paquete Poppr y el software STRUCTURE. Resultados. Se obtuvo alto polimorfismo con la amplificación de más de cuatro alelos por locus, presencia de triploidia y elevada diversidad genética con 40 genotipos multilocus únicos, en comparación con otras regiones del país. Las poblaciones se estructuraron de acuerdo a su hospedero con una diferenciación genética (Gst) entre 0.39 y 0.73, probablemente por la preferencia de hospedero y el uso de hospederos alternativos. El análisis de desequilibrio de ligamiento sugiere posible reproducción sexual en poblaciones P. infestans obtenidas de papa y P. betacei de tomate de árbol. Conclusión. El análisis con cinco marcadores microsatélites permitió identificar alto nivel de polimorfismo en las poblaciones de P. infestans sensu lato y alta variabilidad intrapoblacional para el sur de Colombia, así como estructura de las poblaciones de acuerdo a su hospedero.
Citas
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