revista accb, artículos académicos, artículos. biología, ciencias, ACCB, biologicas

Archivos suplementarios

PDF. Pag, 104-117

Palabras clave

Carbonilos
compuestos bioactivos
compuestos fenólicos
oxidación lipídica
TBARS Carbonyls
bioactive compounds
phenolic compounds
lipid oxidation
TBARS

Cómo citar

Villada Ramos, J. A. ., Aguillón Osma, J. ., & Loango Chamarro, N. . (2024). Potencial antioxidante del extracto de hoja de Coffea arabica sobre la lipoperoxidación y oxidación proteica en un modelo de músculo bovino. REVISTA DE LA ASOCIACION COLOMBIANA DE CIENCIAS BIOLOGICAS, 1(36), 104–117. https://doi.org/10.47499/revistaaccb.v1i36.313

Resumen

La calidad de los alimentos es crucial, especialmente en la industria cárnica, donde la oxidación de lípidos y proteínas afecta la calidad sensorial y nutricional. Este estudio evaluó el potencial antioxidante de los extractos metanólico (EM) e hidroalcohólico (EHA) de hojas de Coffea arabica en músculo bovino para mitigar la oxidación lipídica y proteica. Se empleó el método de extracción por maceración, se evaluaron los contenidos de fenoles y flavonoides, y la inhibición del radical DPPH. Los extractos se aplicaron a muestras de músculo bovino, incubadas con un agente oxidante (AAPH), y se evaluaron las formaciones de TBARS, carbonilos proteicos y las modificaciones de proteínas mediante SDS-PAGE. Los resultados mostraron que el EM presenta una mayor concentración de compuestos fenólicos y flavonoides, así como una mejor capacidad antioxidante en comparación con el EHA, con un porcentaje de inhibición del DPPH de 70.4 % y una IC50 de 630 µg/mL, frente a 64.2 % y 732 µg/mL del EHA. Además, el tratamiento con AAPH incrementó significativamente la formación de grupos carbonilo en un 152.5 %, mientras que el EM mostró un aumento del 42.5 % y el EHA del 62.5 %. En cuanto a la peroxidación lipídica, el EM redujo significativamente los niveles de TBARS en comparación con el AAPH, demostrando su capacidad para inhibir la oxidación. En conclusión, los extractos de C. arabica ofrecen una alternativa natural prometedora a los antioxidantes sintéticos, con una notable capacidad para proteger los productos cárnicos de la degradación oxidativa.

https://doi.org/10.47499/revistaaccb.v1i36.313

Citas

Geng L, Liu K, Zhang H. Lipid oxidation in foods and its implications on proteins. Vol. 10, Frontiers in Nutrition. 2023.

https://doi.org/10.3389/fnut.2023.1192199

Silva FAP, Estévez M, Ferreira VCS, Silva SA, Lemos LTM, Ida EI, et al. Protein and lipid oxidations in jerky chicken and consequences on sensory quality. LWT. 2018 Nov 1;97:341-8.

https://doi.org/10.1016/j.lwt.2018.07.022

Torrico DD, Hutchings SC, Ha M, Bittner EP, Fuentes S, Warner RD, et al. Novel techniques to understand consumer responses towards food products: A review with a focus on meat. Meat Sci. 2018 Oct 1;144:30-42.

https://doi.org/10.1016/j.meatsci.2018.06.006

Huang X, Ahn DU. Lipid oxidation and its implications to meat quality and human health. Vol. 28, Food Science and Biotechnology. 2019.

https://doi.org/10.1007/s10068-019-00631-7

Viedma-Poyatos Á, González-Jiménez P, Langlois O, Company-Marín I, Spickett CM, Pérez-Sala D. Protein lipoxidation: Basic concepts and emerging roles. Vol. 10, Antioxidants. 2021; 10(2), 295.

https://doi.org/10.3390/antiox10020295

Zhao J, Chen J, Zhu H, Xiong YL. Mass spectrometric evidence of malonaldehyde and 4-hydroxynonenal adductions to radical-scavenging soy peptides. J Agric Food Chem. 2012;60(38).

https://doi.org/10.1021/jf3026277

Siró I, Kápolna E, Kápolna B, Lugasi A. Functional food. Product development, marketing and consumer acceptance-A review. Vol. 51, Appetite. 2008.

https://doi.org/10.1016/j.appet.2008.05.060

Nguyen TTK, Laosinwattana C, Teerarak M, Pilasombut K. Potential antioxidant and lipid peroxidation inhibition of Phyllanthus acidus leaf extract in minced pork. Asian-Australas J Anim Sci. 2017;30(9).

https://doi.org/10.5713/ajas.17.0036

Caleja C, Barros L, Antonio AL, Oliveira MBPP, Ferreira ICFR. A comparative study between natural and synthetic antioxidants: Evaluation of their performance after incorporation into biscuits. Food Chem. 2017 Feb 1;216:342-6.

https://doi.org/10.1016/j.foodchem.2016.08.075

Caleja C, Barros L, Antonio AL, Ciric A, Soković M, Oliveira MBPP, et al. Foeniculum vulgare Mill. as natural conservation enhancer and health promoter by incorporation in cottage cheese. J Funct Foods. 2015 Jan 1;12:428-38.

https://doi.org/10.1016/j.jff.2014.12.016

Nissen LR, Byrne D V., Bertelsen G, Skibsted LH. The antioxidative activity of plant extracts in cooked pork patties as evaluated by descriptive sensory profiling and chemical analysis. Meat Sci. 2004 Nov 1;68(3):485-95.

https://doi.org/10.1016/j.meatsci.2004.05.004

Shah MA, Bosco SJD, Mir SA. Plant extracts as natural antioxidants in meat and meat products. Meat Sci. 2014 Sep 1;98(1):21-33.

https://doi.org/10.1016/j.meatsci.2014.03.020

Hayes JE, Stepanyan V, Allen P, O'Grady MN, Kerry JP. Evaluation of the effects of selected plant-derived nutraceuticals on the quality and shelf-life stability of raw and cooked pork sausages. LWT. 2011 Jan 1;44(1):164-72.

https://doi.org/10.1016/j.lwt.2010.05.020

Cangeloni L, Bonechi C, Leone G, Consumi M, Andreassi M, Magnani A, et al. Characterization of Extracts of Coffee Leaves (Coffea arabica L.) by Spectroscopic and Chromatographic/Spectrometric Techniques. Foods. 2022 Aug 18;11(16):2495

https://doi.org/10.3390/foods11162495

Patay ÉB, Bencsik T, Papp N. Phytochemical overview and medicinal importance of Coffea species from the past until now. Asian Pac J Trop Med. 2016 Dec;9(12):1127-1135.

https://doi.org/10.1016/j.apjtm.2016.11.008

Murthy PS, Naidu MM. Recovery of Phenolic Antioxidants and Functional Compounds from Coffee Industry By-Products. Food Bioproc Tech. 2012;5(3).

https://doi.org/10.1007/s11947-010-0363-z

Campos-Esparza MR, Sánchez-Gómez MV, Matute C. Molecular mechanisms of neuroprotection by two natural antioxidant polyphenols. Cell Calcium. 2009;45(4).

https://doi.org/10.1016/j.ceca.2008.12.007

Campa C, Mondolot L, Rakotondravao A, Bidel LPR, Gargadennec A, Couturon E, et al. A survey of mangiferin and hydroxycinnamic acid ester accumulation in coffee (Coffea) leaves: biological implications and uses. Ann Bot. 2012;110(3).

https://doi.org/10.1093/aob/mcs119

Aguillón Osma J, Maldonado ME, Loango Chamorro N, Arango Varela SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspectivas en Nutrición Humana. 2013;15(1).

https://doi.org/10.17533/udea.penh.17897

Muñoz-Bernal ÓA, Torres-Aguirre GA, Núñez-Gastélum JA, de la Rosa LA, Rodrigo-García J, Ayala-Zavala JF, et al. NUEVO ACERCAMIENTO A LA INTERACCIÓN DEL REACTIVO DE FOLIN-CIOCALTEU CON AZÚCARES DURANTE LA CUANTIFICACIÓN DE POLIFENOLES TOTALES. TIP. 2017;20(2): 23-28

https://doi.org/10.1016/j.recqb.2017.04.003

Armentano MF, Bisaccia F, Miglionico R, Russo D, Nolfi N, Carmosino M, et al. Antioxidant and proapoptotic activities of sclerocarya birrea [(A. Rich.) Hochst.] methanolic root extract on the hepatocellular carcinoma cell line HepG2. Biomed Res Int. 2015;2015.

https://doi.org/10.1155/2015/561589

Egharevba GO, Dosumu OO, Oguntoye SO, Njinga NS, Dahunsi SO, Hamid AA, et al. Antidiabetic, antioxidant and antimicrobial activities of extracts of Tephrosia bracteolata leaves. Heliyon. 2019 Aug 21;5(8):e02275.

https://doi.org/10.1016/j.heliyon.2019.e02275

Bridi R, Giordano A, Peñailillo MF, Montenegro G. Antioxidant effect of extracts from native Chilean plants on the lipoperoxidation and protein oxidation of bovine muscle. Molecules. 2019;24(18).

https://doi.org/10.3390/molecules24183264

Zhou F, Zhao M, Zhao H, Sun W, Cui C. Effects of oxidative modification on gel properties of isolated porcine myofibrillar protein by peroxyl radicals. Meat Sci. 2014;96(4).

https://doi.org/10.1016/j.meatsci.2013.12.001

Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990;186(C).

https://doi.org/10.1016/0076-6879(90)86134-H

Zhou F, Zhao M, Zhao H, Sun W, Cui C. Effects of oxidative modification on gel properties of isolated porcine myofibrillar protein by peroxyl radicals. Meat Sci. 2014;96(4).

https://doi.org/10.1016/j.meatsci.2013.12.001

Wang H, Yang Z, Yang H, Xue J, Li Y, Wang S, et al. Comparative study on the rheological properties of myofibrillar proteins from different kinds of meat. LWT. 2022 Jan 1;153:112458.

https://doi.org/10.1016/j.lwt.2021.112458

Calle-Chumo RN, Calle-Chumo DA, Gallegos-Peredo AS, Jarrín-Oseguera PI. Influence of the Solvent on the Extraction of Phenolic Compounds from Coffee Grounds via Soxhlet Leaching. Ingenieria e Investigacion. 2023;43(1).

https://doi.org/10.15446/ing.investig.97521

Rotundo G, Paventi G, Barberio A, De Cristofaro A, Notardonato I, Russo MV, et al. Biological activity of dittrichia viscosa (L.) greuter extracts against adult sitophilus granarius (l.) (coleoptera, curculionidae) and identification of active compounds. Sci Rep. 2019;9(1).

https://doi.org/10.1038/s41598-019-42886-4

Armijos Moncada DA, Torres Segarra SM. Comparación de la actividad antioxidante de los extractos alcohólico y acuoso de las hojas de Coffea arabica. [Cuenca]: Repositorio Institucional de la Universidad Politécnica Salesiana; 2020.

Kurang RY, Kamengon RY. Phytochemical Test and Antioxidant Activity of Methanol Extract in Arabica Coffee Leaves by Using DPPH Method (1,1-Diphenyl-2-Picrylhydrazyl). Walisongo Journal of Chemistry. 2021;4(2).

https://doi.org/10.21580/wjc.v4i2.8032

Metro D, Cernaro V, Santoro D, Papa M, Buemi M, Benvenga S, et al. Beneficial effects of oral pure caffeine on oxidative stress. J Clin Transl Endocrinol. 2017 Dec 1;10:22-7.

https://doi.org/10.1016/j.jcte.2017.10.001

Stadler RH, Turesky RJ, Müller O, Markovic J, Leong-Morgenthaler PM. The inhibitory effects of coffee on radical-mediated oxidation and mutagenicity. Mutation Research Regular Papers. 1994;308(2).

https://doi.org/10.1016/0027-5107(94)90153-8

Phan DTA, Ha HT, Ho TT. An extract and fractions from Coffea arabica sediment on antioxidant and anti-tyrosinase activities, and on the quality of whiteleg shrimp (Litopenaus vannamei) during refrigerated storage. Prev Nutr Food Sci. 2021;26(3).

https://doi.org/10.3746/pnf.2021.26.3.346

Kim JH, Ahn DU, Eun JB, Moon SH. Antioxidant effect of extracts from the coffee residue in raw and cooked meat. Antioxidants. 2016;5(3).

https://doi.org/10.3390/antiox5030021

do Carvalho DC, Brigagão MRPL, dos Santos MH, de Paula FBA, Giusti-Paiva A, Azevedo L. Organic and Conventional Coffea arabica L.: A Comparative Study of the Chemical Composition and Physiological, Biochemical and Toxicological Effects in Wistar Rats. Plant Foods for Human Nutrition. 2011;66(2).

https://doi.org/10.1007/s11130-011-0221-9

Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-CompartirIgual 4.0.

Derechos de autor 2024 REVISTA DE LA ASOCIACION COLOMBIANA DE CIENCIAS BIOLOGICAS

Descargas

Los datos de descargas todavía no están disponibles.

Métricas

Cargando métricas ...